Effect of two temperatures on in vitro nuclear maturation of bitch oocytes: relation to time culture intervals
Bibliographic record
Abstract
In the present study, two commonly incubation temperatures used on in vitro oocyte maturation in canine species (Canis familiaris) were analysed with the aim to verify the rate of nuclear maturation of bitch oocytes after three culture intervals (48, 72 and 96 h). The culture medium was TCM 199, supplemented with 25mM Hepes/l (v/v), with 10% heat inactivated estrous cow serum (ECS), 50mg/mL gentamicin, 2.2mg/mL sodium bicarbonate and 22mg/mL pyruvic acid, 1.0mg/mL oestradiol (E 8875 Sigma), 0.5mg/mL FSH (Folltropin-V, Vetrepharm Inc. Ont, Canada) and 0.03IU/mL hCG (Profasi HP, Serono, Aubonne, Switzerland). Oocytes were recovered from ovaries of bitches at random estrous cycle stages by routine ovariohysterectomy (n=14) or by therapeutical ovariohysterectomy (n=8) at the veterinary hospital of the Universidade Federal do Rio Grande do Sul (UFRGS), Brazil. There was no statistical difference in the rate of meiosis of oocytes matured at 37 o C or at 39 o C at any time point. However, the proportion of oocytes that reached the metaphase II (MII) stage at 37 o C after 72 h of in vitro culture (16/123; 13%), tended to be higher (P=0.064), when compared to that matured at the 39 o C (6/148; 4,1%). The results demonstrate that temperatures of 37 o C and 39 o C are equally effective for the in vitro culture of bitch oocytes. Keywords: canine/ oocyte/ in vitro
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.002 | 0.002 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".