Ultrastructure of the<i>Aspergillus nidulans hypA1</i>restrictive phenotype shows defects in endomembrane arrays and polarized wall deposition
Bibliographic record
Abstract
Aspergillus nidulans Eidam (G. Wint.) wild-type hyphal morphogenesis requires the hypA gene product. Like its homolog in Saccharomyces cerevisiae Meyen ex E.C. Hansen, TRS120, hypA encodes a cytoplasmic protein likely associated with endomem branes. hypA is not essential, but hypA1 temperature-sensitive strains grow poorly at restrictive temperatures. In younger cells, endomembrane arrays were aberrant, only sometimes resembling wild type. In older cells, Golgi equivalents were swollen, impacted with electron-dense granules. In hypA1 strains grown at 42 °C, the poorly polarized hyphae lack recognizable Spitzenkörper and have walls at least four-fold thicker than those of wild-type or hypA1 strains grown at 28 °C. At restrictive temperatures, both hyphal width and wall thickness increase markedly in basal regions, suggesting wall deposition is impaired. Septa are thicker than in wild type, but have medial pores and Woronin bodies. Individual nuclei and mitochondria are smaller at 42 °C than at 28 °C, but each collectively occupies similar proportions of the cytoplasm. Mitochondrial cristae are reduced in number and width at 42 °C, possibly compromising metabolic efficiency; in older cells, cristae are widely spaced and randomly inserted. If hypA1 cells grown at 42 °C are shifted to 28 °C, the thickened wall is precisely degraded for growth of wild-type branches, which form within 1 h, suggesting areas of nascent polarity formed at 42 °C require the hypA product for wild-type function.Key words: endomembrane, filamentous fungus, electron microscopy, cell wall, secretion, Saccharomyces TRS120.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".