The presence of a 1-aminocyclopropane-1-carboxylate (ACC) deaminase deletion mutation alters the physiology of the endophytic plant growth-promoting bacterium<i>Burkholderia phytofirmans</i>PsJN
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Bibliographic record
Abstract
The structural gene for 1-aminocyclopropane-1-carboxylate (ACC) deaminase (acdS) from the endophytic plant growth-promoting bacterium Burkholderia phytofirmans PsJN was isolated and used to construct a mutant strain B. phytofirmans YS2 (B. phytofirmans PsJN/Delta acdS), in which an internal segment of the acdS gene was deleted. The mutant YS2 lost ACC deaminase activity as well as the ability to promote the elongation of the roots of canola seedlings. Concomitant with the creation of this deletion mutant, a number of physiological changes were observed in the bacterium, including an increase in indole acetic acid synthesis, a decrease in the production of siderophores and an increase in the cellular level of the stationary-phase sigma factor, RpoS. Introduction of the wild-type acdS gene into the mutant YS2 to construct strain B. phytofirmans YS3 (B. phytofirmans YS2/pRK-AcdS) restored both ACC deaminase activity and plant growth-promotion activity in strain YS3. However, the complemented mutant still showed the above-mentioned physiological changes.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
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Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it