A Sequence Specific PCR Marker for Distinguishing Rice Lines on the Basis of Wild Abortive Cytoplasm from Their Cognate Maintainer Lines
Bibliographic record
Abstract
Ensuring the genetic purity of parental lines and hybrids is a prerequisite to realize their full potential. The cytoplasmic male sterile (CMS) lines that are utilized for developing the popular “three‐line” hybrids, often get contaminated with their isonuclear maintainer lines during CMS line multiplication. Use of such CMS lines in hybrid seed production results in the production of genetically impure hybrid seed. We report the identification of a DNA sequence that is homologous to rice mitochondrial DNA but unique to the Wild Abortive (WA) cytoplasmic male sterile lines of rice (Oryza sativa L.). In a polymerase chain reaction (PCR) using total genomic DNA as a template, oligonucleotide primers based on this unique DNA sequence could amplify a fragment from CMS lines of rice and their hybrids but not from their cognate maintainer lines. In tests on mixed samples of plants containing both CMS and Maintainer lines, this PCR assay was used to correctly predict the genotypes of these plants indicating that it can be used to detect the mixture of maintainer lines in the seed stocks of the CMS line.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.001 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.001 | 0.002 |
| Insufficient payload (model declined to judge) | 0.002 | 0.003 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".