Characterization of a major histocompatibility class II <i>A </i>gene (<i>Clha‐DAA</i>) with an embedded microsatellite marker in Atlantic herring (<i>Clupea harengus </i>L.)
Bibliographic record
Abstract
An Atlantic herring major histocompatibility class II A ( Clha‐DAA ) cDNA sequence has been characterized and was shown to encode a leader peptide, alpha‐1 domain, alpha‐2 domain, connecting peptide, transmembrane and cytoplasmic region. The Clha‐DAA protein sequence has all the characteristics of a teleost class II A protein with conserved cysteines in both the alpha‐1 and the alpha‐2 domains and two potential N‐linked glycosylation sites. Exon 2 sequences encoding the polymorphic alpha‐1 domain from different individuals were analysed and revealed the presence of at least two loci. The Clha‐DAA gene consists of four exons and three short introns. Four unique intron 3 sequences from multiple individuals were obtained and were shown to contain a (TG) n microsatellite sequence. Primers were optimized such that only a single microsatellite locus designated Clha‐DAA‐INTR3 was amplified. Four herring populations from the North Sea and the Baltic Sea were genotyped for Clha‐DAA‐INTR3 . In total, 16 Clha‐DAA‐INTR3 alleles were detected; the distribution of the alleles showed no deviation from Hardy–Weinberg expectation. Levels of genetic differentiation among samples were of similar magnitude as have been reported earlier for neutral microsatellite loci between northern North Sea and Baltic Sea herring populations.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".