Synchronization of the circadian rhythm generator and the effects of glucagon on hypothalamic mouse neurons detected by acoustic wave propagation
Bibliographic record
Abstract
A thickness shear mode acoustic wave sensor has been used to study the reaction of clonal, immortalized hypothalamic murine neurons in response to glucagon and serum shock in a label free, continuous and real time manner under physiological conditions. Two cell lines were examined; these were the mHypoE-38s and the mHypoE-46s. The technique possesses sufficient sensitivity to detect minor neuronal changes and is capable of discerning subtle differences in cellular behaviors under both stimuli. The kinetics and magnitude of the changes observed here are significantly different compared to those instigated upon causing depolarization, cytoskeletal modifications and surface-adhesion specific interaction alterations with the same cells. Interestingly, this technique has the sensitivity and capability of observing all such changes at the neuronal level without the necessity for invasive interrogation. Under the influence of glucagon, the neurons display both short- and long-term changes, in particular the resonant frequency shifts by -23 ± 8 Hz (n = 13, std. dev.) and the motional resistance decays at a rate of approximately 10 Ω h(-1) over a 2 hour interval. The effect of synchronizing the neurons prior to glucagon stimulation did not influence the cellular changes observed. The process of partial and full synchronization of the cells resulted in different responses. For full synchronization, the addition of the serum bolus triggered resonant frequency and motional resistance shifts of +75 Hz and +18.5 Ω respectively, which decayed back to baseline levels after 30 minutes. The duration of this decay closely matched the time required for full synchronization in a separate study. The changes observed for partial synchronization were significantly different from full synchronization as the baseline levels in both resonant frequency and motional resistance were not re-achieved indicative of the cell-sensor system detecting the difference between full and partial synchronization. Preliminary qualitative immunocytochemistry and RT-PCR studies on these cells support the results obtained with the TSM sensor for the glucagon receptor study.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".