Identification of a Novel Apolipoprotein(a)-related Protein from the European Hedgehog ( Erinaceus europaeus )
Bibliographic record
Abstract
We have isolated a cDNA encoding an apo(a)-related protein designated HaRP-1 (Hedgehog apo(a) related protein-1). The HaRP-1 cDNA (2114 bp; corresponding to a 2.6 kb transcript) was isolated from a hedgehog liver cDNA library. The HaRP-1 clone corresponded to an open reading frame of 676 amino acids and contains a signal sequence followed by a preactivation domain and 7 kringle domains which exhibit an average of 57% amino acid identity with hedgehog plasminogen kringle III. We expressed HaRP-1 in human embryonic kidney cells; immunoprecipitation of metabolically-labeled conditioned medium from transfected cells showed the presence of a 74 kDa band corresponding to HaRP-1. Of note, we also observed an approximately 72 kDa species present in hedgehog plasma by western blotting using a human anti-apo(a) monoclonal antibody; we speculate that the 72 kDa plasma species corresponds to HaRP-1. Interestingly, although none of the 7 kringle domains contained a canonical lysine-binding site, we found that recombinant HaRP-1 bound specifically to lysine-Sepharose. It is likely that the evolution of the HaRP-1 gene is coincident with the evolution of hedgehog apo(a), both of which occurred by duplication of the plasminogen kringle III motif. The function of HaRP-1 remains unclear at present, but may constititute a member of the family of apo(a) proteins that functions in the regulation of lysine-dependent proteolysis.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".