<i>Potato aucuba mosaic virus</i> in Potato in New York State
Bibliographic record
Abstract
Solanum tuberosum cv. Elmer's Blue is one of a number of heritage potato accessions maintained at Cornell University that exhibit virus-like symptoms of stunting and a leaf yellowing or a mottle mosaic. Testing of this cultivar by double-antibody sandwich (DAS)-ELISA revealed that it was infected with Potato virus S (PVS) but none of the other common potato viruses screened for in North American potato certification programs (3). Mechanical inoculation of sap from potato cv. Elmer's Blue onto Nicotiana debneyii, N. megalosiphon, N. occidentalis, and N. tabacum produced a range of yellowing and mosaic symptoms (symptomless on N. tabacum), indicating the presence of a transmissible agent, but all these hosts tested negative for PVS. To identify possible viruses, reverse transcription (RT)-PCR assays involving generic primers for different groups of viruses were performed on the potato and the Nicotiana spp. Degenerate primers specific to members of the genus Potexvirus (4) amplified a 600-bp region from the symptomatic potato and N. debneyii. Nucleotide sequencing of the RT-PCR amplified product from potato cv. Elmer's Blue (Genbank Accession No. EF609120) and comparisons with GenBank sequences revealed the amplified sequence as having 91% identity with the genomic sequence of Potato aucuba mosaic virus (PAMV; Accession No. S73580). The presence of this virus in potato cv. Elmer's Blue and N. debneyii was confirmed by PAMV-specific antibodies (Agdia, Inc., Elkhart, IN) in a DAS-ELISA format. PAMV is reported to occur worldwide, but uncommonly, with most descriptive work from Europe (2). While this virus has been studied in North America (1,2), these reports employed virus stocks from Europe under experimental conditions or virus in tubers obtained directly from Europe; to our knowledge, there are no unambiguous reports of PAMV in naturally infected North American potato cultivars. By contrast, the PAMV-infected cultivar in this report is a selection originally from a Canadian grower, and although not grown commercially, it is maintained in garden and field plots in New York and other states. References: (1) R. H. Bagnall. Phytopathology 50:460, 1960. (2) G. F. Kollmer and R. H. Larson. Res. Bull. Agric. Exp. Stn. Univ. Wis. 223:1, 1960. (3) S. A. Slack. Page 61 in: Potato Health Management. The American Phytopathological Society. St. Paul, MN, 1993. (4) R. A. A. van der Vlugt and M. Berendsen. Eur. J. Plant Pathol. 108:367, 2002.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.003 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".