MétaCan
Menu
Back to cohort
Record W2017226908 · doi:10.1080/07060660009501166

High diversity in<i>Curtobacterium flaccumfaciens</i>pv.<i>flaccumfaciens</i>characterized by serology and rep-PCR genomic fingerprinting

2000· article· en· W2017226908 on OpenAlex
John G. McDonald, E. Wong

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.
venuePublished in a venue whose home country is Canada.

Bibliographic record

VenueCanadian Journal of Plant Pathology · 2000
Typearticle
Languageen
FieldAgricultural and Biological Sciences
TopicPlant Pathogenic Bacteria Studies
Canadian institutionsCanadian Food Inspection Agency
FundersUniversity of Sussex
KeywordsPathovarEpitopeSerologyBiologyPolyclonal antibodiesPhylogenetic treeMonoclonal antibodyMicrobiologyPolymerase chain reactionVirologyAntigenGeneticsAntibodyGeneBacteria

Abstract

fetched live from OpenAlex

The diversity found in culture collection strains of Curtobacterium flaccumfaciens pv. flaccumfaciens was characterized by serology and repetitive-sequence-based polymerase chain reaction (rep-PCR). Using strain NCPPB 559 as the reference antigen, immunofluorescence tests with polyclonal and monoclonal antibodies confirmed that no epitope appeared to be common to all strains of this pathovar. Screening of hybridoma cell lines againsl strains of this pathovar, as well as against other members of this genus and species, identified two distinct epitopes. Phylogenetic analysis of the rep-PCR genomic fingerprints of Curtobacter, Clavibacter, and Rathayibacter species, grouped all the C. flaccumfaciens strains and pathovars together, but the C. flaccumfaciens pv. flaccumfaciens strains did not form a single cluster. Rather, they dispersed into two clusters, along with strains of other pathovars of this species. Members of one cluster reacted with one of the monoclonal antibodies, while members of the other did not.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.784
Threshold uncertainty score0.996

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.014
GPT teacher head0.170
Teacher spread0.156 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it