High diversity in<i>Curtobacterium flaccumfaciens</i>pv.<i>flaccumfaciens</i>characterized by serology and rep-PCR genomic fingerprinting
Bibliographic record
Abstract
The diversity found in culture collection strains of Curtobacterium flaccumfaciens pv. flaccumfaciens was characterized by serology and repetitive-sequence-based polymerase chain reaction (rep-PCR). Using strain NCPPB 559 as the reference antigen, immunofluorescence tests with polyclonal and monoclonal antibodies confirmed that no epitope appeared to be common to all strains of this pathovar. Screening of hybridoma cell lines againsl strains of this pathovar, as well as against other members of this genus and species, identified two distinct epitopes. Phylogenetic analysis of the rep-PCR genomic fingerprints of Curtobacter, Clavibacter, and Rathayibacter species, grouped all the C. flaccumfaciens strains and pathovars together, but the C. flaccumfaciens pv. flaccumfaciens strains did not form a single cluster. Rather, they dispersed into two clusters, along with strains of other pathovars of this species. Members of one cluster reacted with one of the monoclonal antibodies, while members of the other did not.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".