Molecular cloning and expression of pheromone‐binding protein1 from the diamondback moth, <i>Plutella xylostella</i>
Bibliographic record
Abstract
Abstract We report on the identification and characterization of a new cDNA encoding the pheromone‐binding protein1 (PxylPBP1) from male antennae of the diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae). The deduced amino acid sequences of PxylPBP1 revealed a mature protein of 164 amino acids, with six cysteine residues in conserved positions relative to other known PBP. The alignment of the mature PxylPBP1 with other Lepidoptera PBP showed high sequence similarity (70–80%) with other full‐length sequences from GenBank. Reverse transcription polymerase chain reaction (PCR) analysis revealed that the PxylPBP1 gene is only expressed in antennae. Real‐time PCR further indicated that the expression of PxylPBP1 gene is higher in male moths, and higher in mated moths regardless of gender. The transcription levels of PxylPBP1 gene of unmated male moths reduced with age, and peaked in 4‐h‐old female moths.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".