The role of Cd-binding proteins and phytochelatins in the formation of resistance to cadmium in the cell lines of Nicotiana plumbaginifolia
Bibliographic record
Abstract
The callus lines of Nicotiana plumbaginifolia with equal resistances to cadmium were obtained under different selective conditions, including without the inhibition of phytochelatin synthesis (the Cd-R line) and with the inhibition of a butionine sulfoximine (the Cd-Ri line). The level of phytochelatin synthesis in the Cd-R and Cd-Ri lines exceeded the control value by fivefold and twofold, respectively. Three cadmium-binding proteins with molecular masses of 41, 34, and 19 kDa were observed mainly in the control line. A common feature of both resistant lines was the expression of cadmium-binding proteins with molecular masses of 40, 34, and 19 kDa. The synthesis of relatively low-molecular cadmium-binding proteins was characteristic of the resistant lines as follows: the line Cd-R expressed the proteins with molecular masses of 12.5, 11.5, and 9 kDa, while the Cd-Ri line expressed those with molecular masses of 13 and 10 kDa. We supposed that both phytochelatins and Cd-binding proteins contribute to the resistance to cadmium in N. plumbaginifolia callus lines, and, additionally, that changes in the synthesis of low-molecular Cd-binding proteins can compensate for the lack of phytochelatins.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".