The effect of azobenzene cross-linker position on the degree of helical peptide photo-control
Bibliographic record
Abstract
Photo-switchable α-helical peptides offer a valuable tool to probe protein-biomacromolecule interactions in a spatiotemporally controlled manner. We synthesized a series of 32 residue peptides (AA32 s) with the core structure Ac-W-(E1A2A3A4R5)6-Q-NH2 and introduced the azobenzene based cross-linker BSBCA via reaction with Cys residues spaced at i, i + 7 intervals in different positions along the helix. UV/Vis measurements show that the composition of the photostationary state as well as thermal relaxation rates do not change considerably with changes in cross-linker position. CD analysis shows that photo-control of helix folding/unfolding is most effective when the cross-linker is targeted to the middle of the peptide so long as this segment has a high intrinsic helical propensity. The largest degree of photo-controlled conformational change occurred when a cross-linked central region of high intrinsic helicity was followed on the C-terminal side by a region of lower intrinsic helicity. This indicates the BSBCA cross-linker can act as a nucleation site for N-to-C propagation of a helix. These data help to guide the choice of cross-linking site in larger peptides and proteins where photo-control of conformation is desired.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".