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Record W2018245974 · doi:10.1080/09553000050176315

Radiation effects in spheroids of cells exposed to alpha emitters

2000· article· en· W2018245974 on OpenAlexaff
D.E. Charlton

Bibliographic record

VenueInternational Journal of Radiation Biology · 2000
Typearticle
Languageen
FieldMedicine
TopicEffects of Radiation Exposure
Canadian institutionsConcordia University
Fundersnot available
KeywordsSpheroidCell survivalCellBiophysicsMonte Carlo methodChemistryPhysicsIn vitroBiologyBiochemistryStatistics

Abstract

fetched live from OpenAlex

PURPOSE: To simulate spheroids of cells and use the spheroid models to establish methods of calculating cell survival following exposure to alpha irradiation from decays of 211At and 213Bi. METHODS: Using a Monte Carlo technique, two models of spheroids of cells were generated in which cells either did not or were allowed to overlap. At 40% packing of the volume it is shown that the two models are equivalent for longer-ranged alpha particles. The overlapping model was used to examine the effects of spheroid size and cell packing on cell survival. Cell survival was also calculated for different distributions of alpha decays such as uniform distribution within the spheroid, external to the spheroid and as a shell on the periphery of the spheroid. RESULTS: Three examples of the cell structure of the spheroids are shown. Detailed calculations show that cell survival decreases from 57% to 37% as the spheroid diameter increases from 75 microm to 225 microm for an average of one 211At decay per cell and 50% packing. Increasing the packing of the cells in the spheroid from 40% to 70% reduces survival from 46% to 26% for 200 microm diameter spheroids for one 211At or 213Bi decay per cell. The presence of small regions of unlabelled cells within the spheroids does not significantly change cell survival. CONCLUSIONS: Monte Carlo programmes generating spheroids of cells and their subsequent use to score cell survival for alpha irradiation should be useful in the design and interpretation of work on spheroids of cells in vitro and the application of such modelling to the study of very small tumours in vivo. Copies of the programmes are available from the author on request.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.589
Threshold uncertainty score0.518

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.005
GPT teacher head0.283
Teacher spread0.278 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations24
Published2000
Admission routes1
Has abstractyes

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