Development of sequence-characterized amplified region (SCAR) markers for resistance gene<i>Pc94</i>to crown rust in oat
Bibliographic record
Abstract
Crown rust, caused by Puccinia coronata f. sp. avenae, is an important disease of oat (Avena sativa). Pyramiding effective resistance from diverse sources is one strategy to provide more durable control of crown rust. The objective of this study was to develop a DNA marker for the resistance gene Pc94 to crown rust derived from the diploid oat Avena strigosa. Amplified fragment length polymorphism generated two DNA fragments that were associated with the resistance gene Pc94 in a F2 population derived from a cross between the rust-susceptible oat 'Calibre' and a near-isogenic hexaploid oat line, S42, possessing Pc94. One of the fragments was successfully converted into two sequence-characterized amplified region (SCAR) markers linked to the Pc94 gene in the Calibre/S42 F2 population. Specificity of the SCAR markers to the Pc94 gene was confirmed in three other populations. The estimated distance between the SCAR markers and Pc94 ranged from 0.9 to 3.4 centi-Morgan. These SCAR markers developed for the Pc94 gene will be valuable tools for pyramiding this gene with other resistance genes to crown rust in marker-assisted breeding programs when crown-rust races with the appropriate virulence combinations are not available to detect the genes being introgressed.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".