274 LAPAROSCOPIC OVUM PICK-UP IN SHEEP AND GOATS: EFFECTS OF REPEATED RECOVERIES AND FOLLICULAR DIAMETER
Bibliographic record
Abstract
Laparoscopic ovum pickup (LOPU) is a modern technique that may be used in programs of sheep and goat in vitro embryo production. The effects of repeated recoveries and follicular diameter on the efficiency of LOPU were evaluated in these species. In six Merino donor sheep and five Criolla goats, ovarian stimulation for follicular development was performed by administering a single dose of 60 mg NIH-FSH-P1 (Folltropin®; Bioniche, Canada) and 300 UI of eCG (Novoromon 5000®; Syntex, Argentina). Every four days, a total of 4 LOPU in sheep and 3 in goats were performed 24 h after each ovarian stimulation treatment. The intravaginal sponge with 60 mg of medroxyprogesterone (Esponjas Vaginales Syntex®; Syntex, Argentina) were removed after the last LOPU. Follicular fluid was aspirated under laparoscopic observation according to the follicle diameter with a cannula for intrauterine artificial insemination without a vacuum pump. The number and size of follicles, oocyte quantity and quality and recovery rate were evaluated. A total of 566 follicles were aspirated (14.4 ± 2.4 follicles/sheep and 14.6 ± 7.5 follicles/goat), yielding a total of 263 oocytes (6.8 ± 1.5 oocytes/sheep and 6.6 ± 2.9 oocytes/goat) and a recovery rate of 47% in sheep and 46% in goats (Table 1). In both species, there were no significant differences in the number of aspirated follicles, recovered oocytes, overall recovery rate, and the recovery rate of good quality oocytes in the repeated LOPU. The oocyte recovery rates in sheep differed significantly according to the follicular size (55% in large follicles, 36% in small follicles; P < 0.05), whereas in goats recovery rates of oocytes related to follicular size showed no significant differences (42 and 54% for large and small follicles, respectively). Follicular sizes from which oocytes were obtained was not a determining factor in the recovery rate of good quality oocytes. The technique used would allow effective and repeated oocyte recoveries for in vitro embryo production in sheep and goats. Table 1. Repeated recoveries by laparoscopic ovum pickup (LOPU) in sheep and goats
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".