Dye-Affinity Partitioning of Acidic, Basic, and Neutral Proteins in Ionic Liquid-Based Aqueous Biphasic Systems
Why this work is in the frame
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Bibliographic record
Abstract
Abstract The partitioning behavior of model acidic (bovine serum albumin, BSA), basic (hen egg white lysozyme, LYS), and neutral (horse heart myoglobin, MYO) proteins in ionic liquid-based aqueous biphasic systems containing Reactive Red-120 dye has been reported. This study found that partition coefficients of the proteins depended on their chemical structures, pH of the aqueous phase, temperature, and composition of the aqueous biphasic system. It was found that partition coefficients of proteins in aqueous biphasic system containing RR-120 were in the order of LYS > MYO > BSA. Recovery of the hydrophilic ionic liquid, [C4mim]Br, was achieved by using hydrophobic ionic liquid, [C4mim]PF6. Keywords: bovine serum albumindye-affinity partitioningionic liquidlysozymemyoglobin ACKNOWLEDGEMENTS The authors wish to acknowledge the support of this work by Shiraz University Research Council.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it