Bibliographic record
Abstract
The objective of this study was to evaluate the use of a second flush for bovine embryo recovery. A total of 319 clinically healthy Holstein cows (247 lactating, 53 dry, 19 nulliparous) with an average age of 5.5 ± 2.5 years were used for this experiment. Superovulation was performed according to a modified method of Baracaldo et al. (2000). On Day 0 (beginning of the experiment), each cow received 3 mg of estradiol-17ß intramuscularly (i.m.) and a progesterone-releasing vaginal insert (1.9 g of progesterone, CIDR, Pfizer Animal Health, Kirkland, QC, Canada) at random stages of the estrous cycle. From Day 4 evening to Day 8 evening, the cows received a total of 380 mg of NIH-FSH-P1 (FolltropinV, Bioniche Animal Health) administered im through 9 injections of decreasing dose (from 70 to 20 mg) at 12-h intervals. On Day 7, the cows received 2 injections consisting of 500 µg of cloprostenol (prostaglandin F2a analogue Estrumate, Schering-Plough, Pointe-Claire, QC, Canada) given approximately 12 h apart and vaginal inserts were removed 12 h after the last injection. Artificial insemination was performed on Day 10 after treatment with 100 µg, GnRH im (Cystorelin, Merial Canada Inc, Baie d’Urfe, QC, Canada). Embryos were flushed from the uterus of donor cows 6 days after AI. The method consisted of using simultaneously 1 catheter (18Fr Silicone 2-way, Bioniche Animal Health) per uterine horn. Catheters were maintained in place to perform 2 flushes 1 h apart. A total of 1000 mL of flushing media (Complete flush, Bioniche Animal Health) were used, 700 mL and 300 mL for the first and the second flush, respectively. Embryos were assessed for viability immediately after collection using IETS classification. Data were analyzed using the SAS MIXED procedure (SAS Institue, Cary, NC). The mean (±SD) number of embryos collected at the first flushing was 5.87 ± 5.1, 0.92 ± 2.2 and 2.9 ± 4.4 for transferable, degenerate and unfertilized oocytes, respectively. The second flushing yielded 2.32 ± 2.6 transferable embryos, 0.28 ± 0.83 dead embryos and 1.2 ± 2.2 unfertilized oocytes. There was no significant effect of age, day in milk, or stage of lactation on transferable or degenerate embryos or nonfertilized oocytes in each flushing. The embryo recovery method used in this experiment could be used to recover more transferable embryos. The authors want to thank Dr Vincent Girard for his help in statistics.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".