Proliferative Responses by Immune Murine Popliteal Lymph Node Cells to High-Molecular-Weight Basic Allergen of Ryegrass Pollen and Its Cyanogen Bromide Fragments
Bibliographic record
Abstract
High-molecular-weight basic allergen (HMBA) of ryegrass pollen and its fragmented form obtained by cleavage with cyanogen bromide (CNBr) (HMBA-frag) were evaluated for their immunogenicity in terms of their capacity to stimulate the in vitro proliferative responses of the in vivo antigen-primed popliteal lymph node (PLN) cells. For this purpose, mice (C3H/HeJ) were immunized in the hind foot pads with solutions containing 1-5 micrograms of HMBA or HMBA-frag emulsified in Freund's complete adjuvant (FCA). Eleven days later, single-cell suspensions of the PLN were cultured in vitro for 4 days in the presence of either HMBA or HMBA-frag at concentrations ranging from 0.625 to 20 micrograms/ml. 3H-thymidine was added to the cultures 6 h prior to harvesting the cells. The degree of proliferation was assessed from the extent of intracellular incorporation of the 3H-thymidine by the PLN cells. Comparable degrees of lymphocyte proliferation were consistently obtained on stimulation with either HMBA or HMBA-frag of PLN cells from mice immunized with the allergen HMBA. A similar finding was made using PLN cells from mice immunized with the HMBA-frag. This study demonstrated that the CNBr-derived fragments of HMBA retained the immunogenicity of the parent molecule HMBA in terms of being able to (i) induce the in vivo priming of PLN cells and (ii) elicit the in vitro proliferative response of the antigen-primed PLN cells.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".