Characterization and identification of disintegrins in <i>Crotalus</i> <i>horridus </i>venom by liquid chromatography and tandem matrix-assisted laser desorption ionization - quadrupole ion trap time-of-flight (MALDI-QIT-TOF) mass spectrometry
Bibliographic record
Abstract
Two disintegrins were purified from the venom of Crotalus horridus by multiple-dimension liquid chromatography (MDLC) (specifically C18 reverse phase (RP) high-performance liquid chromatography followed by size exclusion chromatography (SEC), and anion exchange chromatography (SCX)). Both disintegrins were subjected to mass spectrometry to determine their intact molecular mass, the number of disulfide linkages, and protein sequence, respectively. These disintegrins were named horrdistatin 1 (nominal mass, 7231 Da) and horrdistatin 2 (nominal mass, 7451 Da), and had IC 50 (inhibitory concentrations) of 12.5 and 16.2 nmol/L (at 50%), respectively. For sequence confirmation from the C-terminal end, both disintegrins were derivatized using chemical-assisted fragmentation (CAF) and subsequently unzipped via collision-induced dissociation (CID) by matrix-assisted laser desorption ionization quadrupole ion trap time-of-flight (MALDI-QIT-TOF) mass spectrometry.Key words: disintegrins, mass spectrometry, snake venom, Timber rattlesnake, Crotalus horridus.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".