Skin prick tests to multiple pollens and prevalence of IgE specific to profilin
Bibliographic record
Abstract
Panallergens like profilin are proteins that have very similar sequences and structure. People can develop IgE specific to these highly cross reactive panallergens. The purpose of this study was to determine the prevalence of profilin sensitivity and the contribution to multiple allergen skin test responses. Sensitivity to profilin was established by IgE binding to purified profilins. Possible effects on skin test results were studied by including Queen Palm (QP) allergen in the skin prick testing panel as a possible source of profilin. QP is a species with a low probability of pollen exposure. 65 subjects from Central Texas and Northern California were recruited for SPT with Multi-Test®II to a panel of up to 51 allergens. Several extracts were tested for profilin and spanned a range of 0 – 14 microgram/mL. QP containing 10ug/mL profilin was added to the panel as a possible source of panallergen. IgE to various purified component major allergens and profilin was determined using Thermo Fisher ISAC microarray or ADVIA Centaur. subjects from Central Texas and Northern California were recruited for SPT with Multi-Test®II to a panel of up to 51 allergens. Several extracts were tested for profilin and spanned a range of 0 – 14 microgram/mL. QP containing 10ug/mL profilin was added to the panel as a possible source of panallergen. IgE to various purified component major allergens and profilin was determined using Thermo Fisher ISAC microarray or ADVIA Centaur. 23 of 65 subjects (35%) had a positive IgE to at least one profilin. Of these, 9 had a >3mm wheal to QP. 20 of 65 subjects (32%), had a positive QP SPT, a similar prevalence of positive SPT as either Timothy grass (43%) or birch (34%) pollen. Subjects reacting by SPT to QP also reacted to multiple allergens, 21 – 100% of allergens tested (average 58%). The multiple allergen SPT sensitivity (percent of 16 allergens) correlated with in vitro profilin IgE binding, QP SPT+, sIgE+, 75%; QP SPT+, sIgE-, 56%;QP SPT-, sIgE+, 40%; QP SPT-, sIgE-, 32%. of 65 subjects (35%) had a positive IgE to at least one profilin. Of these, 9 had a >3mm wheal to QP. 20 of 65 subjects (32%), had a positive QP SPT, a similar prevalence of positive SPT as either Timothy grass (43%) or birch (34%) pollen. Subjects reacting by SPT to QP also reacted to multiple allergens, 21 – 100% of allergens tested (average 58%). The multiple allergen SPT sensitivity (percent of 16 allergens) correlated with in vitro profilin IgE binding, QP SPT+, sIgE+, 75%; QP SPT+, sIgE-, 56%;QP SPT-, sIgE+, 40%; QP SPT-, sIgE-, 32%. Prevalence of IgE to profilin in the current study is similar to reports from European studies. The association of IgE specific to profilin and Queen Palm pollen skin responses suggests this low probability allergen may be useful to identify panallergen responses responsible for multiple sensitivities.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.003 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".