Effects of Cannabinoids on Macromolecular Synthesis in Isolated Spermatogenic Cells
Bibliographic record
Abstract
Effects of delta 9-tetrahydrocannabinol (THC), cannabinol (CBN) and cannabidiol (CBD) (1, 3 or 5 microM for 2 h) on macromolecular synthesis were investigated in homogeneous populations of pachytene spermatocytes and round spermatids obtained from CD-1 mice. Incorporation of 3H-uridine into the acid-insoluble fraction was used as an index of RNA synthesis. Treatment of pachytene spermatocytes with 5 microM THC, CBN and CBD reduced radioactive incorporation by 81%, 62% and 67%, respectively, compared to vehicle (dimethylsulfoxide) controls. Treatment of round spermatids with 5 microM THC, CBN and CBD reduced radioactive incorporation by 54%, 61% and 63%, respectively, compared to controls. In addition to reduction of radioactive incorporation into the acid-insoluble fraction, there was also reduction in the acid-soluble fraction not comparable in magnitude to the reduction in the acid-insoluble fraction. Pachytene spermatocytes and round spermatids isolated from THC-treated mice (10 and 20 mg/kg) showed no reduction in macromolecular synthesis compared to cells isolated from vehicle-treated mice. Suppression of macromolecular synthesis induced by in vitro cannabinoid treatment is discussed in terms of membrane-mediated interference with cell metabolism.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".