Expression of <i>cgmpg2</i>, an Endopolygalacturonase Gene of <i>Colletotrichum gloeosporioides</i> f. sp. <i>malvae</i>, in Culture and during Infection of <i>Malva pusilla</i>
Bibliographic record
Abstract
Abstract A novel polygalacturonase gene was found in the plant pathogenic fungus, Colletotrichum gloeosporioides f. sp. malvae, which causes anthracnose disease of round‐leaved mallow (Malva pusilla). Sequence comparisons showed that this gene, cgmpg2, is highly similar to clgpg2, which was previously described from C. lindemuthianum. In culture, expression of cgmpg2 was observed by reverse transcriptase (RT)‐polymeraase chain reaction (PCR) with mallow cell wall extract as a sole carbon source and a starting pH of 3.5, but not with this substrate at a starting pH of 7.5 or in media with pectin or glucose as sole carbon sources. The expression of cgmpg2 during infection of mallow leaves was compared with the constitutively expressed actin gene, actA, of C. gloeosporioides f. sp. malvae by co‐amplification in RT‐PCR. Expression of cgmpg2 was detected in both the biotrophic and necrotrophic phases of infection with the highest expression levels at the early part of the necrotrophic phase.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".