ANTIOXIDANT EFFECT AND DNA PROTECTIVE EFFECT OF VARIOUS ENZYMATIC EXTRACTS FROM <i>PERILLA FRUTESCENS </i>VAR. <i>CRISPA</i>
Bibliographic record
Abstract
ABSTRACT The antioxidant activity of enzymatic extracts from Perilla frutescens var. crispa was evaluated using 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) radical, hydroxyl radical and alkyl radical‐scavenging activity by employing an electron spin resonance methodology. P. frutescens var. crispa was enzymatically hydrolyzed by eight carbohydrases (Dextrozyme, AMG, Promozyme, Maltogenase, Termamyl, Viscozyme, Celluclast, BAN [Novo Co., Novozyme Nordisk, Bagsvaerd, Denmark]) and nine proteases (Flavourzyme, Neutrase, Protamex, Alcalase, PP‐trypsin [trypsin from porcine pancreas; Novo Co.], papain, pepsin, α‐chymotrypsin, BP‐trypsin [trypsin from bovine pancreas; Sigma Chemical Co., St. Louis, MO]). The DPPH radical‐scavenging activities of Dextrozyme and Neutrase extracts from P. frutescens var. crispa were the highest and the IC50 values were 60.12 and 112.81 µg/mL, respectively. All enzymatic extracts of P. frutescens var. crispa scavenged hydroxyl radical and the scavenging activity increased in a dose‐dependent manner; the IC50 values of Viscozyme and BP‐trypsin, which showed the highest activity, were 0.78 and 0.26 mg/mL, respectively. In addition, the Termamyl and Protamex extracts of P. frutescens var. crispa showed the highest alkyl radical‐scavenging activities, and the IC50 values were 248.13 and 415.23 µg/mL, respectively. The Viscozyme and BP‐trypsin extracts from P. frutescens var. crispa showed protective effect against H2O2‐induced DNA damage. These results indicate that enzymatic extracts of P. frutescens var. crispa possess antioxidant activity. PRACTICAL APPLICATIONS Perilla frutescens var. crispa could be used to produce protein and carbohydrate extracts with antioxidative activeity. Many industrial commercial enzymes, such as Promozyme, Celluclast 1.5 L FG, Maltogenase L, Viscozyme L, Termamyl SC, Dextrozyme E, AMG 300 L, Protamex, Flavourzyme 500 MG, Neutrase 0.8 L, Puncreatic Trypsin and Alcalase 2.4 L, could also be used to attain the extracts processing the high antioxidative activity. The extracts can be used as natural antioxidants.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".