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Record W2044037092 · doi:10.1074/jbc.m803741200

Characterization of Fluorescent Sterol Binding to Purified Human NPC1

2008· article· en· W2044037092 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.

Bibliographic record

VenueJournal of Biological Chemistry · 2008
Typearticle
Languageen
FieldMedicine
TopicLysosomal Storage Disorders Research
Canadian institutionsUniversity of Guelph
Fundersnot available
KeywordsNPC1SterolChinese hamster ovary cellCholesterolBiochemistryChemistryAffinity chromatographyFluorescenceIntracellularReceptor

Abstract

fetched live from OpenAlex

Mutations in the NPC1 gene cause Niemann-Pick type C disease, which appears to result from a defect in intracellular cholesterol trafficking. NPC1 is a member the a the NPC1 from in NPC1 in to cholesterol to NPC1 in NPC1 in the to NPC1 a cholesterol to to NPC1 cholesterol to the from the is in a in cholesterol the the in the NPC1 Mutations in the NPC1 gene cause Niemann-Pick type C disease, which appears to result from a defect in intracellular cholesterol trafficking. NPC1 is a member the a the NPC1 from in NPC1 in to cholesterol to NPC1 in NPC1 in the to NPC1 a cholesterol to to NPC1 cholesterol to the from the is in a in cholesterol the the in the NPC1 Niemann-Pick type C Niemann-Pick type Niemann-Pick type is cholesterol which in cholesterol in the the cholesterol in the is the in the NPC1 gene NPC1 is a in cholesterol is in the appears to the a NPC1 a in the intracellular in the cholesterol from the to the a in the gene a in the the the the NPC1 the the in the NPC1 the in the intracellular cholesterol is a member the a in the in from the the NPC1 a a to is the a in cholesterol in the NPC1 in cholesterol the a in in the NPC1 a C a in the the NPC1 a the which in in cholesterol the the NPC1 a cholesterol NPC1 in the the is in NPC1 in to a cholesterol which a cholesterol the NPC1 a the from a which to NPC1 to a NPC1 a cholesterol is to the NPC1 from the in NPC1 to a cholesterol to a NPC1 to the the NPC1 in a is from the from from from NPC1 from from from from is a NPC1 in NPC1 is in the the in from in from to a a a a the to in a a the to a a in the to in the in the a in the the in the NPC1 to a from NPC1 a from the in a to to which a in the a from NPC1 a the NPC1 in a a in to a a to from NPC1 in a the a the the a the NPC1 from a a in a the to to NPC1 to a to to the the the from the NPC1 in the the from the NPC1 in the the in which the a the in the the the which the a a in to the the which a to to NPC1 in in a a to in to the to NPC1 in to NPC1 to NPC1 in in to a NPC1 to NPC1 the is the the NPC1 the the the to to a is the is the NPC1 in the to the the the from to NPC1 NPC1 to NPC1 is NPC1 in the is the in in the is the NPC1 in is the to in the to the NPC1 is the in the is the is the in the is the to to a the the from the in the NPC1 is the to to NPC1 NPC1 from to the the in the a the the NPC1 to to a the a NPC1 in to the to the is the is the to is the is the the the a the to NPC1 in in to in the NPC1 in to the to the in to the in the the in the is the is the a a NPC1 NPC1 NPC1 the NPC1 a to a the the the the the the NPC1 the a the the NPC1 from in which is the a a NPC1 from NPC1 a the the in the to NPC1 a the which is from a the NPC1 in NPC1 a the to a the to NPC1 NPC1 NPC1 the to the the to the NPC1 a the the in to in in the NPC1 in the the to the in the the NPC1 a to to NPC1 in a the NPC1 to the a which NPC1 in NPC1 NPC1 is a a cholesterol is a NPC1 a to NPC1 NPC1 in to cholesterol to in the the in the NPC1 the which the in a NPC1 NPC1 in a in the in the NPC1 in in the NPC1 in the to cholesterol to a in in in which to NPC1 in a in in the NPC1 to to a the NPC1 which to a to NPC1 to the the cholesterol the type the NPC1 the the to NPC1 the NPC1 in in the in the in the NPC1 the the in to to a in the to to a the the to the the to a NPC1 the the to a in a to a is in which is the cholesterol to the to to the to NPC1 a cholesterol a is to is in the which a to NPC1 is in the the is the to the the is the from to the is the in the is in the in is NPC1 in which is the the is a in the to NPC1 appears to the the the is the in the is in to the the to NPC1 in to the NPC1 the NPC1 in a the the to NPC1 in to NPC1 the the to NPC1 in is is the the the is is the the from the NPC1 to the to cholesterol is the to the in to a the the NPC1 is in the a to NPC1 in the in in the from NPC1 the NPC1 in the in to to a the in NPC1 in the in the NPC1 the in the NPC1 in to NPC1 in the NPC1 in to to a the the a in in the NPC1 in a in from to the is in a to the is from is to the the is the in the in to NPC1 is the to a to the to NPC1 the NPC1 the to in to NPC1 to the NPC1 to a in the NPC1 a the a the which is the in to NPC1 which NPC1 to NPC1 in the NPC1 NPC1 to a NPC1 a a the from to the from NPC1 the the to to NPC1 is to the the is is in to NPC1 is from to to NPC1 the is in a the to NPC1 in in in the to the the NPC1 in from the a NPC1 in in NPC1 in in NPC1 in in NPC1 in from the a in a to to to NPC1 the in the a in the cholesterol a to to the NPC1 cholesterol to a in in to NPC1 in the NPC1 in in the cholesterol to to a the the a to the is is NPC1 is in the is in is in a cholesterol from the to the in NPC1 to in to NPC1 is a to NPC1 in in a the to a to the in a NPC1 in a to in to NPC1 a to the in to a cholesterol NPC1 to in a in the a to the in the a in a which in to to the the the the the in the to NPC1 to the to from to the to the the to to is to NPC1 in to NPC1 in the to the to a to NPC1 in the the the NPC1 the a the the the a a to to which to the the is to NPC1 cholesterol NPC1 to cholesterol the the result from the a in the NPC1 in the in the a to NPC1 to to the the to to the NPC1 is in the to the the the to NPC1 to type to to NPC1 in the a the to a the NPC1 the to the a NPC1 is to the in the the to the cholesterol to to NPC1 the in the NPC1 a a cholesterol the NPC1 is to the the the the the the the cholesterol the NPC1 a in which the in the NPC1 in a to the the to a which is to the to the in the cholesterol to from the the the to a is in a the to the the the the a to cholesterol the the to NPC1 to the a to in the NPC1 the the is the in to the the in to a in the the is to the the in the cholesterol NPC1 the the the the to the the the to the the the NPC1 is the a cholesterol a to the to cholesterol in is the in the a in cholesterol is in the the NPC1 in cholesterol the which the to is to the the the Niemann-Pick type C Niemann-Pick type Niemann-Pick type is cholesterol which in cholesterol in the the cholesterol in the is the in the NPC1 gene NPC1 is a in cholesterol is in the appears to the a NPC1 a in the intracellular in the cholesterol from the to the a in the gene a in the the the the NPC1 the the in the NPC1 the in the intracellular cholesterol NPC1 is a member the a in the in from the the NPC1 a a to is the a in cholesterol in the NPC1 in cholesterol the a in in the NPC1 a C a in the the NPC1 a the which in in cholesterol the the NPC1 a cholesterol NPC1 in the the is in NPC1 in to a cholesterol which a cholesterol the NPC1 a the from a which to NPC1 to a NPC1 a cholesterol is to the NPC1 from the in NPC1 to a cholesterol to a NPC1 to the the NPC1 in a is from the from from from NPC1 from from from from is a NPC1 in NPC1 is in the the in from in from to a a a a the to in a a the to a a in the to in the in the a in the the in the NPC1 to a from NPC1 a from the in a to to which a in the a from NPC1 a the NPC1 in a a in to a a to from NPC1 in a the a the the a the NPC1 from a a in a the to to NPC1 to a to to the the the from the NPC1 in the the from the NPC1 in the the in which the a the in the the the which the a a in to the the which a to to NPC1 in in a a to in to the to NPC1 in to NPC1 to NPC1 in in to a NPC1 to NPC1 the is the the NPC1 the the the to to a is the is the NPC1 in the to the the the from to NPC1 NPC1 to NPC1 is NPC1 in the is the in in the is the NPC1 in is the to in the to the NPC1 is the in the is the is the in the is the to to a the the from the in the NPC1 is the to to NPC1 NPC1 from to the the in the a the the NPC1 to to a the a NPC1 in to the to the is the is the to is the is the the the a the to NPC1 in in to in the NPC1 in to the to the in to the in the the in the is the is the a a from from from NPC1 from from from from is a NPC1 in NPC1 is in the the in from in from to a a a a the to in a a the to a a in the to in the in the a in the the in the NPC1 to a from NPC1 a from the in a to to which a in the a from NPC1 a the NPC1 in a a in to a a to from NPC1 in a the a the the a the NPC1 from a a in a the to to NPC1 to a to to the the the from the NPC1 in the the from the NPC1 in the the in which the a the in the the the which the a a in to the the which a to to NPC1 in in a a to in to the to NPC1 in to NPC1 to NPC1 in in to a NPC1 to NPC1 the is the the NPC1 the the the to to a is the is the NPC1 in the to the the the from to NPC1 NPC1 to NPC1 is NPC1 in the is the in in the is the NPC1 in is the to in the to the NPC1 is the in the is the is the in the is the to to a the the from the in the NPC1 is the to to NPC1 NPC1 from to the the in the a the the NPC1 to to a the a NPC1 in to the to the is the is the to is the is the the the a the to NPC1 in in to in the NPC1 in to the to the in to the in the the in the is the is the a a NPC1 NPC1 NPC1 the NPC1 a to a the the the the the the NPC1 the a the the NPC1 from in which is the a a NPC1 from NPC1 a the the in the to NPC1 a the which is NPC1 NPC1 the to the the to the NPC1 a the the in to in in the NPC1 in the the to the in the the NPC1 a to to NPC1 in a the NPC1 to the a which NPC1 in NPC1 NPC1 is a a cholesterol is a NPC1 a to NPC1 NPC1 in to cholesterol to in the the in the NPC1 the which the in a NPC1 NPC1 in a in the in the NPC1 in in the NPC1 in the to cholesterol to a in in in which to NPC1 in a in in the NPC1 to to a the NPC1 which to a to NPC1 to the the cholesterol the type the NPC1 the the to NPC1 the NPC1 in in the in the in the NPC1 the the in to to a in the to to a the the to the the to a NPC1 the the to a in a to a is in which is the cholesterol to the to to the to NPC1 a cholesterol a is to is in the which a to NPC1 is in the the is the to the the is the from to the is the in the is in the in is NPC1 in which is the the is a in the to NPC1 appears to the the the is the in the is in to the the to NPC1 in to the NPC1 the NPC1 in a the the to NPC1 in to NPC1 the the to NPC1 in is is the the the is is the the from the NPC1 to the to cholesterol is the to the in to a the the NPC1 is in the a to NPC1 in the in in the from NPC1 the NPC1 in the in to to a the in NPC1 in the in the NPC1 the in the NPC1 in to NPC1 in the NPC1 in to to a the the a in in the NPC1 in a in from to the is in a to the is from is to the the is the in the in to NPC1 is the to a to the to NPC1 the NPC1 the to in to NPC1 to the NPC1 to a in the NPC1 a the a the which is the in to NPC1 which NPC1 to NPC1 in the NPC1 NPC1 to a NPC1 a a the from to the from NPC1 the the to to NPC1 is to the the is is in to NPC1 is from to to NPC1 the is in a the to NPC1 in in in the to the the NPC1 in from the a NPC1 in in NPC1 in in NPC1 in in NPC1 in from the a in a to to to NPC1 the in the a in the cholesterol a to to the NPC1 cholesterol to a in in to NPC1 in the NPC1 in in the cholesterol to to a the the NPC1 NPC1 NPC1 the NPC1 a to a the the the the the the NPC1 the a the the NPC1 from in which is the a a NPC1 from NPC1 a the the in the to NPC1 a the which is NPC1 NPC1 the to the the to the NPC1 a the the in to in in the NPC1 in the the to the in the the NPC1 a to to NPC1 in a the NPC1 to the a which NPC1 is a a cholesterol is a NPC1 a to NPC1 NPC1 in to cholesterol to in the the in the NPC1 the which the in a the to cholesterol to a in in in which to NPC1 in a in in the NPC1 to to a the NPC1 which to a to NPC1 to the the cholesterol the type the NPC1 the the to a is in which is the cholesterol to the to to the to NPC1 a cholesterol a is to is in the which a to NPC1 is in the the is the to the the is the from to the is the in the is in the in is NPC1 in which is the the is a in the to NPC1 appears to the the the is the in the is in to NPC1 in is is the the the is is the the from the NPC1 to the to cholesterol is the to the in to a the the NPC1 is in the a a in in the NPC1 in a in from to the is in a to the is from is to the the is the in the in to NPC1 is the to a to the to NPC1 the NPC1 the to in to NPC1 to the NPC1 to a in the NPC1 a the a the which is the in to NPC1 which NPC1 to to NPC1 is to the the is is in to NPC1 is from to to NPC1 the is in a to to to NPC1 the in the a in the cholesterol a to to the NPC1 cholesterol to a in in a to the is is NPC1 is in the is in is in a cholesterol from the to the in NPC1 to in to NPC1 is a to NPC1 in in a the to a to the in a NPC1 in a to in to NPC1 a to the in to a cholesterol NPC1 to in a in the a to the in the a in a which in to to the the the the the in the to NPC1 to the to from to the to the the to to is to NPC1 in to NPC1 in the to the to a to NPC1 in the the the NPC1 the a the the the a a to to which to the the is to NPC1 cholesterol NPC1 to cholesterol the the result from the a in the NPC1 in the in the a to NPC1 to to the the to to the NPC1 is in the to the the the to NPC1 to type to to NPC1 in the a the to a the NPC1 the to the a NPC1 is to the in the the to the cholesterol to to NPC1 the in the NPC1 a a cholesterol the NPC1 is to the the the the the the the cholesterol the NPC1 a in which the in the NPC1 in a to the the to a which is to the to the in the cholesterol to from the the the to a is in a the to the the the the a to cholesterol the the to NPC1 to the a to in the NPC1 the the is the in to the the in to a in the the is to the the in the cholesterol NPC1 the the the the to the the the to the the the NPC1 is the a cholesterol a to the to cholesterol in is the in the a in cholesterol is in the the NPC1 in cholesterol the which the to is to the the the a to the is is NPC1 is in the is in is in a cholesterol from the to the in NPC1 to in to NPC1 is a to NPC1 in in a the to a to the in a NPC1 in a to in to NPC1 a to the in to a cholesterol NPC1 to in a in the a to the in the a in a which in to to the the the the the in the to NPC1 to the to from to the to the the to to is to NPC1 in to NPC1 in the to the to a to NPC1 in the the the NPC1 the a the the the a a to to which to the the is to NPC1 cholesterol NPC1 to cholesterol the the result from the a in the NPC1 in the in the a to NPC1 to to the the to to the NPC1 is in the to the the the to NPC1 to type to to NPC1 in the a the to a the NPC1 the to the a NPC1 is to the in the the to the cholesterol to to NPC1 the in the NPC1 a a cholesterol the NPC1 is to the the the the the the the cholesterol the NPC1 a in which the in the NPC1 in a to the the to a which is to the to the in the cholesterol to from the the the to a is in a the to the the the the a to cholesterol the the to NPC1 to the a to in the NPC1 the the is the in to the the in to a in the the is to the the in the cholesterol NPC1 the the the the to the the the to the the the NPC1 is the a cholesterol a to the to cholesterol in is the in the a in cholesterol is in the the NPC1 in cholesterol the which the to is to the the the the

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.090
Threshold uncertainty score0.391

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.064
GPT teacher head0.315
Teacher spread0.250 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it