Comparing methods of preservation for cultures of entomopathogenic fungi
Bibliographic record
Abstract
Nine species of entomopathogenic fungi were tested for viability after they had been stored with deionized water, mineral oil, or silica gel or frozen at 20 or 80°C. Species tested included members of the Hyphomycetes, Entomophthorales, Trichomycetes, and Oomycetes. The fungal cultures were maintained up to 1.5 years and were checked at 3, 6, 12, and 18 months. For all species evaluated, Paecilomyces fumosoroseus demonstrated the best results, surviving through 18 months when stored with water or mineral oil and when frozen at 80°C. For the majority of other fungal species tested, except the trichomycete Smittium culisetae and oomycete Leptolegnia chapmanii, freezing at 80°C was the best storage method and storage with silica gel was the worst. In addition to culture viability, infectivity against Aedes aegypti larvae was evaluated after 18 months of storage for L. chapmanii and S. culisetae. The simplest and least expensive methods, using water or mineral oil, were the only successful methods for maintaining viability and infectivity of L. chapmanii and S. culisetae.Key words: entomopathogenic fungi, preservation, storage, viability.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.002 | 0.002 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.001 | 0.001 |
| Open science | 0.001 | 0.001 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".