Spatial and temporal deposition of suberin during maturation of the onion root exodermis
Bibliographic record
Abstract
Suberin is a complex biopolymer composed of a poly(aliphatic) domain (SPAD) and a poly(phenolic) domain (SPPD). Suberin is typically confined to specialized cell types including root exodermal cells, but its synthesis in a maturing exodermis is still not well understood. For the current work, Allium cepa roots were used as a model to analyze SPAD and SPPD synthesis in a maturing uniseriate exodermis. Roots were divided into four maturation zones based on their growth rate and the deposition of suberin lamellae in maturing exodermal cells. Exodermal and epidermal cell layers were separated from the underlying layers in each maturation zone, then soluble and insoluble suberin monomers were extracted chemically, and quantified and identified by gas chromatography – mass spectrometry. Temporal patterns for the synthesis of the SPAD, but not for the SPPD, were revealed upon resolution of the metabolite profiles. The composition of the soluble fraction was essentially unchanged as the exodermis matured. In contrast, the SPAD composition differed during maturation, mainly owing to significant increases in the deposition of C18:1 α,ω-dioic acid and ω-OH fatty acids. It is proposed that the exodermal maturation zones with their corresponding metabolite profiles be used as targets for the functional enzymatic characterization of suberin biosynthetic pathways.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".