Autolysis and biochemical properties of endogenous proteinases in Japanese sandfish (<i>Arctoscopus japonicus</i>)
Bibliographic record
Abstract
Summary Autolysis of Japanese sandfish ( Arctoscopus japonicus ) was studied. The highest autolysis was observed at 60 °C. Optimal pH for autolysis was found at both acidic and alkaline pH values. Autolytic activity decreased with increasing NaCl concentration. Further assay of the extract from Japanese sandfish exhibited the maximum activity at 55 °C and pH 9.5. The Japanese sandfish proteinases were markedly inhibited by pepstatin A at pH 3.5, while soybean trypsin inhibitor, PMSF and TLCK inhibited activities of proteinases at pH 9.5, suggesting Japanese sandfish proteinases were both aspartic and serine proteinases. The extracts were stable to heat treatment up to 50 °C for 15 min and still remained high relative activity after incubated at 50 °C for up to 8 h. Maximum stability of the extracts was observed between pH 6 and 11 and no changes of activity were found after incubation at pH 9.5 for up to 8 h. Activity of Japanese sandfish extract continuously decreased as NaCl concentration increased (0–30%), while activity increased as CaCl 2 concentration increased. The results suggest that major proteinases in Japanese sandfish were trypsin‐like serine proteinases.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".