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ORAL ARTIFICIAL CELLS BIOENCAPSULATED GENETICALLY ENGINEERED CELLS CAN LOWER UNWANTED ELECTROLYTES IN UREMIC RATS

2001· article· en· W2046982168 on OpenAlexaffabout
Satya Prakash, T. M. S. Chang

Bibliographic record

VenueASAIO Journal · 2001
Typearticle
Languageen
FieldMedicine
TopicPancreatic function and diabetes
Canadian institutionsMcGill University
Fundersnot available
KeywordsUric acidIn vitroIn vivoInternal medicineOral administrationAlkaline phosphataseEndocrinologyChemistryCreatinineUremiaMoleElectrolyteMedicineBiologyBiochemistryEnzymeBiotechnology

Abstract

fetched live from OpenAlex

A) Purpose of study: We have shown earlier that oral administration of genetically engineered cells can lower urea, uric acid, and creatinine in uremic rats. The present study is to see whether this can lower K+, P, Cl−, and P in uremic rats. B) Methods used: Genetically engineered E. coli DH5 cells are encapsulated in artificial cells. The efficacy for the removal of electrolytes was analyzed in-vitro in batch bioreactors and in-vivo in daily oral administration to partially nephrectomized male Wistar rats. C) Results summary: In-vitro: Significant lowering of plasma K+, P, Na+, and Cl− occurs in in-vitro experiments in batch bioreactors. In-vivo experiments in uremic rats show that oral administration also resulted in lowering of plasma electrolytes levels. For example, plasma K+ decreases from 5.66 + 1.15 (mmol/L) to 4.23 ± 0.90 (mmol/L), plasma P from 2.44 ± 0.56 (mmol/L) to 1.54 ± 0.59 (mmol/L), plasma Na+ 146 ± 6.30 (mmol/L) to 131.20 ± 8.60 (mmol/L), plasma Cl− from 206.00 ± 49.70 (mmol/L) to 101.00 ± 15.00 (mmol/L), and alkaline phosphatase from 212.33 ± 63.21 (u/l) to 87.66 ± 24.54 (u/l). On discontinuation of the therapy, electrolyte levels increased again. Ref.: www.artcell.mcgill.ca

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesInsufficient payload (model declined to judge)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.019
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.001
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.013
GPT teacher head0.236
Teacher spread0.224 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2001
Admission routes2
Has abstractyes

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