Rhizosphere-competent isolates of streptomycete and non-streptomycete actinomycetes capable of producing cell-wall-degrading enzymes to control<i>Pythium aphanidermatum</i>damping-off disease of cucumber
Bibliographic record
Abstract
Fifty-eight streptomycete and 35 non-streptomycete actinomycetes were isolated from cucumber rhizosphere soil. These isolates were screened for the production of cell-wall-degrading enzymes using mycelial ( Pythium aphanidermatum (Edson) Fitzp.) fragment agar. Eighteen promising isolates were screened for their competence as root colonizers. Eight isolates showing exceptional rhizosphere competence significantly inhibited, in vitro, P. aphanidermatum, the causal agent of postemergence damping-off of cucumber ( Cucumis sativus L.) seedlings. The four most inhibitory isolates ( Actinoplanes philippinensis Couch, Microbispora rosea Nonomura and Ohara, Micromonospora chalcea (Foulerton) Ørskov, and Streptomyces griseoloalbus (Kudrina) Pridham et al.) produced in vitro β-1,3-, β-1,4-, and β-1,6-glucanases and caused lysis of P. aphanidermatum hyphae. None of these produced volatile inhibitors or siderophores. Only S. griseoloalbus produced diffusible inhibitory metabolites, whilst A. philippinensis and Micromonospora chalcea parasitized the oospores of P. aphanidermatum. These four isolates were subsequently tested in the greenhouse, individually or as a mixture, for their ability to suppress damping-off of cucumber seedlings in soil with or without cellulose amendment. The treatment, which included all four isolates in soil amended with cellulose, was significantly superior to all other treatments in suppressing damping-off and was nearly as good as the metalaxyl treatment. Results show that there is a potential to use a mixture of antagonistic rhizosphere-competent actinomycetes along with cellulose amendment rather than fungicides for the field management of this disease. This is the first study that has involved the screening of rhizosphere-competent non-streptomycete actinomycetes capable of producing cell-wall-degrading enzymes, for the management of Pythium diseases.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".