227 SUPEROVULATION AND EMBRYO COLLECTION IN WOOD BISON DURING THE ANOVULATORY SEASON
Bibliographic record
Abstract
Brucellosis and tuberculosis are endemic in Wood Buffalo National Park, the largest reserve of wood bison (Bison bison athabascae) in Canada. Our goal is to produce and preserve disease-free embryos for the purpose of wood bison repopulation. This study was designed to determine if embryo collection is feasible in wood bison during the anovulatory season (May–July) and to test if progesterone priming is required for superovulation. A 2-by-2 design was used to determine the effectiveness of LH (Lutropin) or hCG (Chorulon) for induction of ovulation with or without intravaginal progesterone releasing device (PRID) in 32 wood bison cows. Follicular wave emergence was synchronized among bison by transvaginal ultrasound-guided follicle ablation. Synchronized bison were assigned to 4 groups: PRID+LH (n = 12), PRID+hCG (n = 4), no-PRID+LH (n = 12) and no-PRID+hCG (n = 4). A PRID was inserted on the day of follicular ablation in the respective groups. A single SC dose of 400 mg FSH (Folltropin) in a slow-release formulation was given the day after follicular ablation (i.e. on the expected day of a new follicular wave emergence, Day 0). The PRID was removed on Day 4 and either 25 mg LH or 2000 IU hCG was given IM on Day 5. Artificial insemination was done at 24, 36 and 48 h after LH or hCG treatment. Embryos were collected nonsurgically on Day 13 using commercial bovine equipment. Transrectal ultrasonography was done on Days 0, 5, 6, 7, 8 and 13 to record follicular and ovulatory responses. Count data (mean ± SEM) were analysed by two-way ANOVA and proportions by chi square. The number of ovulatory-sized follicles (=10 mm) on Day 5 did not differ among groups (P = 0.33; Table 1). Ovulation rate (number of ovulations/number of follicles =10 mm) was greater in bison treated with hCG (P < 0.05; Table 1). The number of corpora lutea (CL) on Day 13 was greater in bison treated with hCG without a PRID (P < 0.05; Table 1). No differences in number of ova/embryos and transferable embryos were found among groups (P = 0.36 and P = 0.52, respectively; Table 1). In conclusion, progesterone priming (PRID) had no effect on ovarian superstimulation in wood bison in the anovulatory season. The ovulatory response was satisfactory only in bison treated with hCG. Embryo collection is feasible in wood bison, but the reasons for a low embryo collection rate in all groups remain unclear. Table 1. Response to superovulation and embryo collection in wood bison Funded by Advancing Canadian Agriculture and Agri-Food and Agri-Food Innovation.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".