Cloning and characterization of a gene coding for a class I chitin synthase from <i>Fusarium graminearum</i>
Bibliographic record
Abstract
The full-length sequence of a gene coding for a class I chitin synthase (Chs) from Fusarium graminearum (Fg) was isolated by genome walking, using gene-specific primers and walking libraries constructed with a panel of eight restriction enzymes. Analysis of the gene (named FgChs1) and its corresponding cDNA revealed an open reading frame of 2703 bp interrupted by two introns of 120 and 55 bp, respectively. FgChs1 is a single-copy gene. Its deduced amino acid sequence shows 77% identity to Neurospora crassa Chs3 and 73% identity to Ophiostoma novo-ulmi ChsA in the conserved central region (residues 153 to 800). Quantitative reverse transcription – polymerase chain reaction was used to compare the levels of FgChs1 mRNA at different stages of development. This showed that FgChs1 was developmentally regulated, with peaks of expression during spore germination and mycelial growth. The first significant increase in FgChs1 mRNA levels was detected after 1 day of hyphal growth while the second peak was apparent after 5 days. This result suggests that FgChs1 may play an important role in fungal growth and development.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".