Effect of High Hydrostatic Pressure on the Secondary Structures of BSA and Apo‐ and Holo‐α‐Lactalbumin Employing Fourier Transform Infrared Spectroscopy
Bibliographic record
Abstract
ABSTRACT Conformational changes in bovine serum albumin (BSA), Ca ++ ‐free α‐lactalbumin(apo‐α‐lac), and Ca ++ ‐saturated α‐lactalbumin(holo‐ct‐lac) were examined via Fourier transform infrared (FTIR) spectroscopy after pressure treatment up to 1270 MPa. BSA showed minimal irreversible secondary structural changes with pressurization, showing a slight loss of β‐sheet structure in favor of α‐helix. Apo‐α‐lac underwent major irreversible changes and exhibited infrared bands characteristic of aggregation upon decompression. Holo‐α‐lac showed extensive resistance to high pressure with no major structural changes. The rich disulfide content in BSA and the presence of Ca ++ in holo‐α‐lac may play important roles in stabilizing these proteins against pressure denaturation.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".