Characterization of lipoxygenase extracts from <i>Penicillum</i> sp.
Bibliographic record
Abstract
Abstract Biomasses of Penicillium camemberti and P. roqueforti strains were grown and harvested after 10 d of incubation, a period that corresponded to the maximal dry weight of mycelium as well as to lipoxygenase (LOX) activity. Partially purified LOX extracts were obtained by ammonium sulfate precipitation of the crude enzymatic extracts that had been recovered from the biomasses. The partially purified LOX extracts exhibited a preferential specificity toward free fatty acids, including linoleic, linolenic, and arachidonic acids, rather than fatty acid acylglycerols, including mono‐, di‐, and trilinolein. The K m and V max values of LOX activity were investigated. Normal‐phase high‐performance liquid chromatography (HPLC) and gas chromatography/mass spectrometry analyses showed that the LOX activity of the microbial extracts converted linoleic acid mainly into the corresponding 9‐ and 13‐hydroperoxides (HPOD). However, the production of a significant proportion of 10‐HPOD, ranging from 4 to 9% of the total HPOD, was also demonstrated. In addition, the enantiomeric ratios of the 9‐ and 13‐HPOD produced were determined at both pH optima by chiral‐phase HPLC. The results indicated that an almost racemic mixture had been obtained which can be related to either low enantioselectivity of LOX or the presence of isozymes showing complementary enantioselectivity.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".