Influence of Temperature on Survival and Conjugative Transfer of Multiple Antibiotic-Resistant Plasmids in Chicken Manure and Compost Microcosms
Bibliographic record
Abstract
The aim of this study was to determine if mobile plasmids carrying antibiotic-resistant genes could survive and be transferred in chicken manure maintained under conditions similar to those found in commercial cage layer operations and during composting. Escherichia coli J5 harboring a self-transmissible plasmid (RP4) and E. coli C600 harboring a mobile plasmid (pIE723) were used as plasmid donors; E. coli CV601 was used as a plasmid recipient. At 23 degrees C both plasmids were transferred to E. coli CV601 in chicken manure and in compost microcosms that consisted of a mixture of chicken manure and peat. The transfer frequencies ranged from 8.1 x 10(-5) to 2.4 x 10(-3) per donor cell in manure and from 2.4 x 10(-5) to 5.5 x 10(-4) per donor cell in compost microcosms. After 45 d of incubation at 23 degrees C, RP4, but not pIE723, was recovered by an exogenous isolation method although their E. coli hosts were not cultured from the microcosms. However, when the temperatures of the compost microcosms were elevated to 50 degrees C or above, neither the plasmids nor their E. coli hosts could be detected. The results suggested that composting of chicken manure at high temperatures could help prevent the spread of antibiotic-resistant genes via plasmids in the environment.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.003 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".