Effect of mustard rhizobacteria on wheat growth promotion under cadmium stress: characterization of acdS gene coding ACC deaminase
Why this work is in the frame
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Bibliographic record
Abstract
Ten mustard rhizobacterial isolates that utilize 1-aminocyclopropane-1-carboxylate (ACC) as sole nitrogen source were screened for plant growth-promoting traits. These isolates enhanced root elongation significantly and minimized ethylene synthesis in wheat seedlings under induced cadmium stress condition. The presence of acdS gene coding for ACC deaminase was tested through gene-specific PCR amplification and further confirmed by nucleic acid hybridization. acdS gene from isolates Ps 2-3 and Ps 7-12 selected on the basis of wheat growth promotion was cloned and sequenced. Sequence analysis showed an open reading frame of 1017 bp encoding a complete polypeptide with an identity of 86.4 % between each other and sharing 84–95 % similarity with the earlier reported acdS genes. The deduced protein sequences were highly conserved and shared a 95.2 % homology at the amino acid level. Eight and thirteen new amino acid residues were found in the protein sequence of Ps 2-3 and Ps 7-12 with variation in numbers of different peptidase acting sites and protein motifs. Based on 16S rDNA sequencing, the isolates Ps 2-3 and Ps 7-12 were identified as Pseudomonas sp. and Pseudomonas fluorescens , respectively.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it