221 PREPUBERTAL MOUSE BIOASSAY FOR OVULATION-INDUCING FACTOR IN SEMINAL PLASMA
Bibliographic record
Abstract
A substance in the seminal plasma of llamas and alpacas has been discovered that induces ovulation and growth of the corpus luteum (CL) in the female of the same species. The ovarian effects of the ovulation-inducing factor (OIF) are associated with a surge release of LH into circulation. Ultrasonographic detection of ovulation and CL development is currently the only method available for testing the bioactive effects of OIF. The purpose of this study was to determine if a superstimulatory prepubertal mouse model could be developed as an in vivo bioassay for OIF. Prepubertal female CD1 mice ( n = 144), 20 days of age and weighing 20–25 g, were housed at 24°C with lights on from 0500 to 1900 h and free access to food and water. An intramuscular dose of 5 IU of eCG (Novormon, Bioniche Animal Health, Belleville, ON, Canada) was given (Day 0) for ovarian superstimulation. On Day 2, mice were assigned randomly to 4 groups ( n = 36 per group) and given a single 0.1 mL intraperitoneal dose of (1) 5 IU of hCG (Chorulon, Intervet Canada, Ltd., Whitby, ON, Canada), (2) 5 µg GnRH (gonadotropin-releasing hormone: Cystorelin, Merial, Ltd., Iselin, NJ, USA), (3) llama seminal plasma, or (4) phosphate-buffered saline (negative control). On Day 3, females were euthanized by an overdose of inhaled halothane. Oviducts were collected and oocytes were counted using trans-illumination stereomicroscopy. The proportion of mice that ovulated did not differ among groups treated with hCG, GnRH, and seminal plasma (31/36, 31/36, 28/36, respectively); however, the proportion of mice that ovulated in each treatment group was greater than that in the saline-treated group (9/36) ( P < 0.001). The number of oocytes counted (mean ± SEM) was also similar among groups treated with hCG (25.8 ± 2.9), GnRH (27.4 ± 2.7), and seminal plasma (19.2 ± 2.8), all of which were greater ( P < 0.01) than in the saline-treated group (6.2 ± 2.1). We conclude that the superstimulated prepubertal CD1 mouse model is effective as an in vivo bioassay for OIF in seminal plasma. Whether the bioassay may be used for quantitative estimates of OIF activity will require dose-response trials using serial dilutions of seminal plasma.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.002 | 0.001 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.001 | 0.002 |
| Insufficient payload (model declined to judge) | 0.003 | 0.002 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".