Camptothecin production in <i>Camptotheca acuminata</i> cultured hydroponically and with nitrogen enrichments
Bibliographic record
Abstract
Camptothecin (CPT), produced by Camptotheca acuminata Decaisne (Nyssaceae), is becoming a therapeutically important compound, and the demand for CPT is increasing dramatically. Feasibility of CPT production from C. acuminata in hydroponic culture was studied. As nitrogen (N) is required for the biosynthesis of the alkaloid CPT, CPT production in C. acuminata was studied in response to enrichments of 7.5, 15.0, 22.5, or 30.0 mM N in the culture medium. High concentrations of CPT were found in leaves of plants in hydroponic culture, but the N concentration in the culture medium did not affect tissue CPT concentration, plant weight, plant height, leaf number, lamina dry weight per area, total stem weight, total leaf weight, or total leaf CPT yield (the product of leaf CPT concentration and total leaf dry weight per plant). However, increasing the N concentration in the culture medium reduced leaf length and total root weight. We conclude that C. acuminata CPT production in hydroponic culture is feasible in terms of efficiency for CPT production, and is a potential alternative to culture in the field or in pots, or cell or callus culture, and that a standard low N rate in the culture medium is recommended. Key words: Anti-cancer compound, HPLC, medicinal plant
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".