Use of a 15N-tracer Method as a Tool to Indicate the Assimilation of Elicitin-Sterol Complexes by Phytophthorasojae
Bibliographic record
Abstract
The mechanism of sterol acquisition and transport in oomycetes of the genus Phytophthora is suspected to involve secreted class-I elicitins, but little information is available to confirm this. The objective of this study was to determine if class-I elicitins of Phytophthorasojaepromote assimilation into this organism when a phytosterol is amended into its growth medium using a qualitative 15N tracer method. Two P. sojae growth experiments were conducted involving 15N labeled mycelium or 15N spent broth containing putative P. sojaeelicitins in the absence or presence of a phytosterol (stigmasterol). Mycelium was harvested after 0, 2, 3 or 6 days of growth and analyzed for d15N by Elemental Analysis-Isotopic Ratio Mass Spectrometry (EA-IRMS). Results from the two experiments demonstrated that P. sojae assimilated putative elicitins from the spent broth more rapidly when stigmasterol was present in the growth medium. These results provide evidence for the involvement of secreted class-I elicitins in sterol acquisition by P. sojae.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".