Molecular Cloning and Characterization of a Putative BnHEC3 Gene in Oilseed Rape (Brassica Napus)
Bibliographic record
Abstract
HECATE3 (HEC3) is an important transcription factor involved in regulating the carpel and transmitting tractdevelopment in Arabidopsis thaliana. The homologous gene of HEC3 in Brassica napus (designed as BnHEC3) wasobtained by in silico cloning and rapid amplification of cDNA end (RACE) method. BnHEC3 obtained from cDNA is of748 bp, containing an open reading frame (ORF) of 546bp and coding a peptide of 181 amino acid residues. Sequencealignment revealed that BnHEC3 is highly homologous to HEC3 in Arabidopsis thaliana, especially in bHLH region,which is sharing nearly 100% identity, and the polygenetic analysis indicated that BnHEC3 belongs to atypical bHLHprotein family. RT-PCR results showed that BnHEC3 is mainly expressed in young leave. Southern blotting analysisindicated that BnHEC3 has at least three copies in Brassica napus genome. The transient expression of BnHEC3-sGFPin onion epidermic cells showed that BnHEC3 localized in nuclei. These features implied that BnHEC3 functions asHEC3, and may be involved in the development of female reproductive tissues in Brassica napus.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".