Pyrene Biodegradation by <i>Mycobacterium Frederiksbergense</i>Using an Encapsulated Oil System
Why this work is in the frame
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Bibliographic record
Abstract
Abstract Polycyclic aromatic hydrocarbons (PAHs) belong to a large group of organic pollutants and are persistent in nature. Negligible bioavailability due to low aqueous phase solubility of these compounds largely limits their biodegradation. The present study investigated oil encapsulation method for delivery and enhancement of bioavailability in biodegradation of PAHs using Mycobacterium frederiksbergense. Using pyrene as the model PAH compound, different organic solvents were tested for their solubilizing ability and biocompatibility with pyrene. Solubility of pyrene in silicone oil, paraffin oil, hexadecane, and isopropyl myristate (IPM) were found to be 2.8, 25.5, 40, and 72 g/l, respectively. Besides, paraffin oil and silicone oil showed relatively high degrees of biocompatibility. However, based on both pyrene solubilizing ability and biocompatibility, paraffin oil was further selected and pyrene-containing paraffin oil was successfully encapsulated within chitosan-coated alginate-polyvinyl alcohol (PVA) microspheres with more than 99% encapsulation efficiency. Nearly complete degradation of 20 g/l pyrene by M. frederiksbergense was achieved within seven days employing the novel paraffin oil encapsulated PAH delivery system. Key Words: alginate-PVA beadbiodegradationencapsulationparaffin oilpyrene Mycobacterium frederiksbergense Acknowledgments The authors gratefully acknowledge the financial support for this research work from the Department of Biotechnology, Government of India, vide sanction no. BT/PR-8844/BCE/08/519/2007.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.001 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it