Pyrene Biodegradation by <i>Mycobacterium Frederiksbergense</i>Using an Encapsulated Oil System
Bibliographic record
Abstract
Polycyclic aromatic hydrocarbons (PAHs) belong to a large group of organic pollutants and are persistent in nature. Negligible bioavailability due to low aqueous phase solubility of these compounds largely limits their biodegradation. The present study investigated oil encapsulation method for delivery and enhancement of bioavailability in biodegradation of PAHs using Mycobacterium frederiksbergense. Using pyrene as the model PAH compound, different organic solvents were tested for their solubilizing ability and biocompatibility with pyrene. Solubility of pyrene in silicone oil, paraffin oil, hexadecane, and isopropyl myristate (IPM) were found to be 2.8, 25.5, 40, and 72 g/l, respectively. Besides, paraffin oil and silicone oil showed relatively high degrees of biocompatibility. However, based on both pyrene solubilizing ability and biocompatibility, paraffin oil was further selected and pyrene-containing paraffin oil was successfully encapsulated within chitosan-coated alginate-polyvinyl alcohol (PVA) microspheres with more than 99% encapsulation efficiency. Nearly complete degradation of 20 g/l pyrene by M. frederiksbergense was achieved within seven days employing the novel paraffin oil encapsulated PAH delivery system.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".