Activity of Lambda-Exonuclease on Surface-Attached Oligonucleotide Detected by Acoustic Wave Device and Radiochemical Labeling
Bibliographic record
Abstract
Abstract Single-strand 25-mer oligonucleotides have been attached to the surface of a 9-MHz acoustic wave sensor incorporated into a flow-through configuration. This was achieved by an online interaction of the biotinylated probe with chemisorbed neutravidin. Duplex formation with complementary 25-mer was monitored by acoustic wave detection in real time. Regeneration of the probe-modified surface was achieved using online introduction of λ-exonuclease, an enzyme that digests a single strand of double-stranded DNA beginning with the 5′ end, cleaving single nucleotides as it progresses. Thus, with the 5′ end of the probe strand remaining inaccessible because of the biotin-neutravidin linkage, the target strand was successfully removed by enzymatic digestion. The results of the acoustic wave work are correlated with analogous experiments involving oligonucleotides labeled with 32P. Keywords: Acoustic wave devicebiosensors λ-exonucleaseflow-through formatnucleic acid 32P labelingsensor regeneration The authors are grateful to the Natural Sciences and Engineering Research Council of Canada for support of this work. Also, we thank Jon Ellis of the Institute of Biomaterials and Biomedical Engineering of the University of Toronto for very helpful discussions regarding the physics of acoustic wave propagation and assistance in the preparation of the manuscript. Notes Note. Additions of labeled F2 are given in bold type. a Labeled F2 is designated F2∗. Conditions are A: F2 (39 pmol), exo (90 min); B: F2 (735 pmol), exo (90 min); C: F2 (750 pmol), exo (15 min); D: F2 (1490 pmol), exo (15) min.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".