RNA polymerase I transcription obstructs condensin association with 35S rRNA coding regions and can cause contraction of long repeat in <i>Saccharomyces cerevisiae</i>
Bibliographic record
Abstract
In many eukaryotic cells, the ribosomal RNA gene (rDNA) is composed of a highly repetitive structure. Previously, we reported the isolation of condensin mutants of Saccharomyces cerevisiae that were defective in carrying long rDNA repeat due to the loss of the replication fork barrier (RFB) protein Fob1p; thus the repeat in the mutants were dramatically contracted. The reintroduction of the FOB1 gene suppressed the contraction of the repeat. It was found that condensin mainly localized at the RFB site in a FOB1-dependent fashion. Here, we show that RNA polymerase I transcription interferes with condensin association with 35S rRNA coding regions in fob1 cells and causes dramatic contraction of rDNA repeat in the fob1 condensin double mutant. Inactivation of RNA polymerase I suppresses the dramatic contraction of the rDNA repeat in the fob1 condensin double mutant. These results suggest that association of condensin with the RFB site outside the active transcription region avoids the dramatic contraction of the rDNA repeat. We also found that the stimulation of RNA polymerase II transcription within the rDNA repeat decreased condensin association with actively transcribed regions. Thus, a characteristic of condensin is that its association with the chromatin is interfered by transcription.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".