Cadmium bioaccumulation and metallothionein induction in the liver of the Antarctic teleost <i>Trematomus bernacchii</i> during an on-site short-term exposure to the metal via seawater
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Bibliographic record
Abstract
Abstract A short-term experiment (7 days) was carried out to study cadmium accumulation and metallothionein (MT) gene expression in the liver of the Antarctic teleost Trematomus bernacchii when exposed to 2.0 mg Cd L−1 seawater. Metal determinations were carried out by differential pulse anodic stripping voltammetry while MT gene expression was determined by the Real Time PCR Detection System. In controls, the Cd concentration and the MT gene expression remained practically constant during the experiment (8.8 ± 0.9 µg g−1 d.w. and 1.0 ± 0.2 mRNA a.u., respectively). In exposed organisms, while Cd increased almost linearly with time, up to 30 ± 2 µg g−1 d.w., i.e. by a factor of ∼3.5 in 7 days, MT gene expression, after a little delay, increased almost exponentially up to a value of 86 ± 25 mRNA a.u. after 7 days, by a factor of ∼90. These results confirm the potential usefulness utility of T. bernacchii as a bioindicator of environmental pollution in Antarctica. Keywords: Trematomus bernacchii Antarcticacadmiumbioaccumulationmetallothioneinsvoltammetry Acknowledgments Financial support from the Italian Programma Nazionale di Ricerche in Antartide under the project on "Chemical Contamination" is gratefully acknowledged. Many thanks are due to the technical personnel of ENEA (Ente Nazionale Energia e Ambiente) at Terra Nova Bay, and to the scientists of 2005–2006 expedition for the experimental activities performed on-site.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.001 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.003 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it