Failure of short-term feed restriction to affect luteinizing hormone and leptin secretion or subcutaneous adipose tissue expression of leptin in the prepuberal gilt
Bibliographic record
Abstract
Previously we reported that 24-h fast increased serum non-esterified fatty acids (NEFA) concentrations, and decreased leptin pulse frequency, but not mean serum leptin concentrations in the OVX prepuberal gilt. The present study was conducted to determine if short-term feed restriction in the prepuberal gilt would reduce BF gene transcript expression, pulsatile LH secretion and serum leptin concentrations. OVX prepuberal gilts 79 ± 4 kg BW were control fed 3 kg (CF; n = 6) or feed restricted (RST; 33% of CF diet; n = 6) for 8 d to determine the effects of RST on leptin and LH secretion, metabolism, leptin, Ob-rb and transcription factor expression in BF. Blood samples were collected on day 8 and BF on day 9. RST gilts lost BW (-2.5 vs. +8.2 kg; SE = 0.6 kg; P < 0.001) and BF (-3 vs. +2 mm; SE = 1mm; P < 0.02) compared with CF gilts. Postprandial serum T4 concentrations increased (P < 0.04) in CF, but not in RST pigs. Postprandial serum insulin and BHBA concentrations were similar between treatments. A treatment × time interaction was detected for plasma glucose (P < 0.02) and NEFA (P < 0.04) concentrations. Feed restriction failed to affect leptin or LH secretion except for LH pulse amplitude was greater (P < 0.01) in RST gilts. RST failed to affect BF leptin, Ob-rb, AFABP, C/EBPα, or PPARγ2 expression. In conclusion, the ability of the prepuberal gilt to maintain energy level in the normal range suggests the prepuberal animal is resistant to moderate reduction in nutrition. Thus, leptin may not play a primary role in the regulation of LH secretion in the prepuberal gilt, but may serve as a metabolic signal reflecting the amount of stored energy, which may be necessary for activation of the reproductive axis during puberal development in the gilt. Key words: Leptin, luteinizing hormone, nutrition, adipocyte, pig
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".