Optimized St. John's Wort (Hypericum perforatum L.) Germplasm Lines Exert Cytotoxicity in HT-29 Colon Cancer Cells via Downregulation of NF-?B
Why this work is in the frame
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Bibliographic record
Abstract
Extracts of two germplasm lines of St. Johns wort (SJW; Hypericum perforatum L.) selected for enhanced hypericin and hyperforin content were evaluated for potential activity against colon cancer. Bioactivity was assessed in signaling pathways of tumor necrosis factor-a (TNF-?) and nuclear transcription factor-?B (NF-?B) in HT-29 colon cancer cells. Both extracts and the hypericin standard significantly inhibited growth of HT-29 cells. Levels of active NF-?B were reduced in cells treated with either of the plant extracts or hypericin, but the purified hyperforin standard was comparatively ineffective. The combination of TNF-? and SJW treatments had significantly higher cytotoxic effects, and reduced the expression of NF-?B, inhibitor of NF-?Bs (I?Ba), I?B kinase b (IKKb), and TNF receptor-1. These observations indicate the potential of SJW as a source of cancer therapies, including those that act synergistically with TNF-?. The phytochemical complexity of SJW tissues necessitates consistent, optimized plant extracts for optimal results and the data indicate that additional, possibly unidentified, phytochemicals from SJW have potential in the treatment or prevention of colon cancer.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it