Enhanced Production of Red Blood Cells in Suspension by Electrostatic Interactions with Culture Plates
Bibliographic record
Abstract
Enucleation of erythroblasts, a critical step in the generation of red blood cells (RBCs), occurs at a low rate without cocultured stromal cells. Previously, the surface properties of the cell culture plate were not considered in the enucleation process, because the cells exist in suspension. Here, we show that a significantly higher rate of enucleation of erythroblasts occurred on the positively charged plates than on the negatively charged surfaces or the both negatively and positively charged plates. Also, the negatively and positively charged plate group showed a significantly higher enucleation than did the hydrophobic plates. Therefore, the plates fully coated with amine groups generated 1.88 times more enucleated RBCs than did the hydrophobic plates. This study suggests an important insight into the effect of surface characteristics of cell culture plates on suspension cell culture. Further, this simple and inexpensive procedure could contribute to a more efficient RBC production system, eliminating the need for robust and expensive coculture procedures.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".