The COMA complex is required for Sli15/INCENP-mediated correction of defective kinetochore attachments
Bibliographic record
Abstract
Before anaphase, kinetochores of sister chromatid (SC) pairs must be attached to microtubules emanating from opposing spindle poles. The conserved Aurora B kinase Ipl1 and its adaptor INCENP/Sli15 correct defective attachments and promote SC bi-orientation. Ame1 and Okp1 are essential components of the COMA sub-complex of the budding yeast central kinetochore, and Ame1 has been demonstrated to physically interact with Sli15. Here, we examine the significance of the Ame1-Sli15 interaction in vivo. We find Sli15 localization at kinetochores is reduced in the absence of Ame1. We demonstrate a role for Ame1 in the correction of defective attachments. While overexpression of OKP1 restores kinetochore localization of the mutant Ame1-4 protein, Sli15 localization is not restored and defective attachments are not corrected. Our findings reveal a new role for the central kinetochore in promoting Sli15 function, and suggest functional Ame1 is required for the correction of defective attachments by promoting the localization of Sli15/INCENP at kinetochores.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.000 | 0.001 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".