<i>Citrus tristeza virus</i> ultrastructure and associated cytopathology in <i>Citrus sinensis</i> and <i>Citrus aurantifolia</i>
Bibliographic record
Abstract
Citrus tristeza virus ultrastructure and associated cytopathology was documented with three isolates and two hosts, sweet orange 'Madam vinous' (Citrus sinensis (L.) Osbeck) and Mexican lime (Citrus aurantifolia (L.) Swingle). Virions were long, flexuous, and disorganized or in swirled, parallel masses. Infection was common in phloem parenchyma and companion cells and less frequent in mature sieve elements. Immunogold labeling confirmed previous findings that the major coat protein encapsidated the length of purified virions, while the minor coat protein encapsidated one terminal. Three types of inclusions were observed: (i) viral arrays that reacted with antibodies against the major (p25) and minor (p27) Citrus tristeza virus coat proteins, (ii) fibrous inclusions that reacted with antibodies against the Citrus tristeza virus p20 gene product but were sparsely labeled with antibodies against either coat protein, and (iii) accumulated cytoplasmic vesicles associated with aggregated, vesiculating mitochondria. The latter resembled Beet yellows virus-like vesicles, which are typical of closterovirus infection, but did not react with any of our antibodies. Cytopathology did not differ between isolates and plant hosts. Most effects were observed in phloem parenchyma cells, including chloroplast degradation, mitochondria vesiculation, and nuclear membrane invagination. Multivesicular bodies and lipid-filled vesicles were abundant in the cytoplasm. Masses of electron-lucent vesicles and electron-dense bodies were present between the cell membrane and cell wall.Key words: immunolocalization, CTV major coat protein, CTV minor coat protein, CTV p20 gene product, inclusions, isolate severity.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".