Design and Development of Light-Sensitive Chitosan-Based Nanocarriers for Gene Delivery
Bibliographic record
Abstract
In this work, we report on the development of a multifunctional and photo-inducible nanoplex made of chitosan (Ch) and hyaluronic acid (HA) for delivery of nucleic acid. Self-assembled Ch/HA nanoparticles were attached to ortho-nitrobenzyl (o-NB) photo-labile molecules (PL)-gold nanoparticles via thiol groups and to QDs-conjugate ssDNA through amide bond linkage to form nanoplexes (Ch:HA:AuPL:QD-DNA). The composition of DNA nanocarriers was validated by nuclear magnetic resonance, transmission electronic microscopy, energy dispersive x-ray spectroscopy, gel electrophoresis and spectrophotometry. The change in zeta potential (34 ± 11 to -26 ± 11 mV) and the loss of the o-NB characteristic peaks in nuclear magnetic resonance spectra, after the exposure of the PL molecule to ultraviolet light, both confirmed the photo-labile properties of the system. The potential of the nanoplexes to induce high cell transfection was assessed by flow cytometry and fluorescent microscopy imaging. Over 30% transfection of HEK-293 was obtained with the nanoplexes after a one-minute exposure of cells to UV light. This corresponds to a 15% increase in the transfection efficiency compared to unexposed Ch:HA:AuPL:QD-DNA nanocarriers. This high transfection efficiency was associated with the unique design of the carrier system and its photo-responsiveness feature for facilitating the DNA release.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".