Molecular cloning and expression analysis of four turmeric MAP kinase genes in response to abiotic stresses and phytohormones
Why this work is in the frame
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Bibliographic record
Abstract
Plant mitogen activated protein kinase (MAPK) cascades comprise a complex network playing a major role in regulating extracellular stimuli as well as developmental processes. The present study involves cloning four MAPKs (ClMPK1, 3, 4 and 5) from Curcuma longa. All four ClMPKs have fully canonical motifs of MAPK and each is represented by a single copy in the turmeric genome. The analysis of exon-intron junctions revealed conserved nature of ClMPKs across different plant groups. The RT-qPCR analysis showed their expression in mature plant tissues. The transcript analysis using the RT-qPCR shows that the four ClMPKs were differentially regulated by cold, salinity, and drought stresses. ClMPK4 showed a significant upregulation in the presence of NaCl, polyethylene glycol, and mannitol. The time-course expression analysis revealed a marked accumulation of ClMPK1 and ClMPK4 transcripts after mechanical wounding or applications of abscisic acid, H2O2, methyl jasmonate, and salicylic acid. ClMPK5 showed a unique and pronounced expression in response to hexavalent chromium (CrVI).
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it